Rotor Gene 6000 Real-Time PCR Machine Features: • Unique centrifugal rotary format • Uniformity: ± 0.01°C • Resolution: 0.02°C (smallest programmable and reported thermal increment) • 5-Plex (Green, Yellow, Orange, Red, Crimson) fluorescence channels • High resolution melt (HRM) application • Holds 36 x0.2ml tubes, 72 x 0.1ml strip tubes or 100 Gene-Disc • Software: Build-in extensive analysis, graphing and statistical functions. Unlimited use software license for Windows XP, Pentium IV (2GHz) or higher PC. Rotor Gene 3000 Real-Time PCR Machine Features: • Unique centrifugal rotary format • Excitation Source: LED high power diodes, 470nm, 530nm, 585nm and 625nm • Detection Filters: 510nm (FAM), 555nm (JOE), 610nm (ROX) and 660nm high-pass (Cy5) • Holds 36 x0.2ml tubes or 72 x 0.1ml strip tubes • Software: Build-in extensive analysis, graphing and statistical functions. Use of the machines in the Molecular Biology Facility • The Rotor-Gene machines are in high demand therefore there is an (external website). Plan ahead, book time slots and stick to them. • Users are responsible for supplying all their own plastic ware and reagents.
0.1ml tubes and Gene-Disc 100 are produced by Corbett especially for the Rotor-Gene. • Save files in your own folder in the 'Rotor Gene Run File' folder in the local disk (C:). The computer is also linked to the network for easy transfer of your data to the server. Tips and addition information • The Rotor-Gene machines support reaction volumes from 5 µl to 100 µl (rotor and protocol dependent). Typical reaction volume is 20µl.
• The use of a normalisation dye, such as ROX reference dye, is optional on the Rotor-Gene. • Many companies supply master mixes for real-time PCR which can decrease the chance of pipetting errors in this highly sensitive method. • If you would like to learn more about the real-time PCR experimental design and data analysis, please contact Dr. Donna Lai, the Molecular Biology Officer. Bioanalyzer, Real-time RT-PCR workshops are conducted occasionally during the year.
Please contact Dr. Donna Lai for details. A Little Jazz Exercise Oscar Peterson Pdf File more.
Free Download: Rotor Gene 6. Published: 2. A rapid prototyping tool to make prototypes faster. BS1 Enterprise Accounting - Free Edition 2. How to get support. Your Corbett warranty is valid at QIAGEN. Telephone: 800-DNA-PREP (800-362-7737) more. Sample to Insight Investor Relations. Free Download: Rotor Gene. Published: 2. A rapid prototyping tool to make prototypes faster. BS1 Enterprise Accounting - Free Edition 2.
QIAGEN's real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications. Is the new operating and analysis software for life science qPCR applications, with a plug-in concept that lets you add new functionality without affecting established workflows. Explore the to learn more about the Rotor-Gene Q. A human A/T SNP in the AHRR7 gene was analyzed using genomic DNA from wild-type (blue), homozygous mutant (green), and heterozygous (red) samples. Experiments were performed using the Type-it HRM PCR Kit and a Rotor-Gene Q cycler with an HRM channel.
Data analysis was performed with the unsupervised mode of Rotor-Gene ScreenClust HRM Software. A/T polymorphisms (class IV SNPs) are most difficult to discriminate due to minute differences between homozygote alleles (in this example, less than 0.1°C). [ A] HRM raw data, [ B] cluster plot.
All pseudo-unknowns were correctly clustered according to genotype. Twofold dilutions of human genomic DNA from 30 ng (10,000 copies) to 0.06 ng (20 copies) were used as a template in real-time PCR. Five replicate reactions were run for each dilution using a self-designed TaqMan® assay for IL1R2 and the Rotor-Gene Probe PCR kit on the Rotor-Gene Q. The average difference in the C T values between all dilutions was 1.07 cycles. Human genomic DNA was used as template in 72 replicate real-time PCRs using a self-designed TaqMan® assay for BCL2 on the Rotor-Gene Q without ROX normalization. The average C T value was 24.94 with a standard deviation of only 0.05, equivalent to a CV of 0.2%. The Rotor-Gene Q is the only real-time cycler currently capable of deciphering the most difficult class IV SNPs by HRM.